FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n01_2602_d8_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n01_2602_d8_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1491037
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAATAGGAGAACCAAAAATATGAAAGAACTGAATATAACTTCTAGGACTA16010.10737493435776578No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA4000.067.0790561
AGCGAAA7150.055.7831
GCGAAAG7900.050.4837572
CGAAAGC8250.048.3420223
GAGCAAA37800.045.6594161
CAAAAGC39700.044.019244
GCAAAAG42250.040.675953
CAATAGG11500.037.2048341
AGCAAAA48950.034.5159072
GTCGGAA850.002742703534.1169177
ATAGGAG14300.031.4392193
AAAAGCA57950.030.2815425
CGGAGTA2202.2619133E-829.6644254
AAAGCAG68800.029.1949256
GCTATTG7750.028.9993748
GAGCTAT8000.028.09886
TATAGCT4900.025.1575834
TCGGAGT2601.1435441E-725.1006643
AAGCAGG83150.024.7619447
GCTATAT6500.024.544521
Produced by FastQC (version 0.11.5)