FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n01_2602_d8_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n01_2602_d8_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3269437
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCAAA82050.050.8164331
GAGCGAA12300.046.5732841
AGCGAAA24400.041.6057321
CGAAAGC25300.040.9842643
GCGAAAG25850.040.112262
CAAAAGC115550.035.5807234
GCAAAAG124650.032.9253
GCTATAT7500.031.905651
GTTACGT5200.030.6785091
AGCAAAA134850.030.219482
ACATGGT23300.029.875625
AAAGCAG159200.029.7876556
AAGCAGG166550.028.5553687
AAAAGCA166200.024.955525
TATATAC17450.024.5164873
CTATATA11850.023.8642562
GTTGACA31050.023.1200371
AGGTCGG15200.022.4172768
TATATCG1952.6139116E-422.310965
AGCAGGT38500.022.2202958
Produced by FastQC (version 0.11.5)