FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n01_2602_d6_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n01_2602_d6_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2078823
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA10350.057.446221
GCGAAAG11800.051.0003972
GAGCGAA5850.050.8178061
GAGCAAA51000.045.495431
CGAAAGC13150.045.2132383
CAAAAGC56400.040.752824
GCAAAAG62600.036.2532963
AGCAAAA72850.031.0529272
GTTACGT2701.72804E-1029.5404341
GACCGGC3700.027.431627145
AAAGCAG96600.026.946036
AAAAGCA87150.026.6231885
AAGCAGG114450.023.1841167
TTACGTG3503.5033736E-922.7877852
GTCGAAG2353.709058E-521.5977083
TACGTGT4001.6323611E-819.9393123
TAGTACT5153.8198777E-1119.710534
TATACTG10850.019.379645
GTACGGA1900.00588606819.0811421
AAGACCG3852.385823E-718.8328825
Produced by FastQC (version 0.11.5)