FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n01_2602_d6_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n01_2602_d6_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3071996
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCAAA74600.046.6552471
GAGCGAA13500.042.4317971
AGCGAAA22900.039.263071
CGAAAGC23000.038.4624673
GCGAAAG23800.038.3882872
ACTCCGA8500.034.1223641
CAAAAGC105450.032.8002324
GTTACGT5100.031.2788371
GCTATAT5850.030.987191
GCAAAAG116550.029.7386153
AAGCAGG141450.028.599987
AAAGCAG149200.027.4104046
AGCAAAA130850.026.3777872
CCGACTA4700.024.6846664
CTATATA9550.023.5376682
GTCGGCT7750.023.3907156
ACATGGT24200.023.0717495
AAAAGCA154300.022.7919245
CCGAGGT13900.022.4315264
TATTGGG14500.022.0034032
Produced by FastQC (version 0.11.5)