FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n01_2602_d2_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n01_2602_d2_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2795094
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA7550.070.105611
GAGCAAA63650.050.236231
AGCGAAA15600.047.4080851
GCGAAAG18050.041.374152
CAAAAGC77700.040.9649664
GCAAAAG78050.040.1309933
CGAAAGC19350.038.594493
GCTATAT12000.033.836361
GTTACGT3350.032.465591
GACCGGC5850.032.221645145
AGCAAAA97950.032.051812
TACCATG14100.031.8794337
AAAAGCA108800.029.4552335
TATATAC20250.029.3601253
AAAGCAG128900.028.8558276
CTATATA15450.028.6266442
GCTATTG10400.027.8836178
AAGCAGG156800.023.858427
GAGCTAT13850.022.5106376
CTGATCG5001.8189894E-1221.7492249
Produced by FastQC (version 0.11.5)