FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n01_2602_d2_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n01_2602_d2_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2914212
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA7900.056.9070321
GAGCAAA67250.049.1670231
CGAAAGC18800.040.1108553
AGCGAAA18600.039.763861
TATATCG2350.037.0254025
GCGAAAG20500.036.4315152
CAAAAGC100800.033.2328724
GCAAAAG100000.032.556133
GCTATAT6900.030.4754561
ACATGGT18950.029.0797625
AAAGCAG133700.028.471766
AAGCAGG134650.028.1588347
GTCGGCT8100.027.7500086
CTATATA8350.026.9196322
AGCAAAA121350.026.7092482
AGTCGAA2507.831841E-826.1033572
TATCGTG3451.09139364E-1025.2163077
AGTCGGC8950.025.114535
TATATAC14200.025.0203933
ACTCCGA8850.024.5798551
Produced by FastQC (version 0.11.5)