FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n01_2602_d12_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n01_2602_d12_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1903484
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA6450.046.0965541
AGCGAAA8750.041.4387131
CGAAAGC9200.039.4097483
GCGAAAG9650.037.571992
GAGCAAA42750.032.9086381
CAAAAGC42950.030.0523434
GCAAAAG47650.028.1533663
TCGGCGC1358.086256E-426.851372145
GTACGGA1851.8284765E-423.519271
AGCAAAA58900.022.2835712
GACCGGC3955.9117156E-1022.024921145
AAAGCAG76150.021.2352076
GTATATA15000.018.8546161
GATATAT18750.018.5645451
AAAAGCA72500.018.4035365
TCGGAGT3953.1200398E-718.357963
AAGCAGG92550.017.3902877
CACGAGC6455.2750693E-1116.860163145
GTATTAT23150.016.6023351
ACGGTTC3504.1653962E-516.571138
Produced by FastQC (version 0.11.5)