FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n01_2602_d12_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n01_2602_d12_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3193871
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAAGC22600.042.0278133
GCGAAAG23800.039.6041152
AGCGAAA25600.035.6865461
GAGCAAA75850.034.9864161
CAAAAGC87350.030.214294
GTATATA17250.027.7414261
GCAAAAG96500.027.0488683
GTTACGT5550.023.515461
AGCAAAA114600.023.0298272
AAAGCAG134200.022.6378636
TATATCG2353.710094E-521.597535
ATAATCG2053.6668233E-421.2212683
ACTCCGA7200.021.1475811
GTATTAT28150.020.863181
TTATACT11600.020.6267054
AAAAGCA132050.020.2610455
AAGCAGG141900.020.232337
TATACTG22950.019.5876775
GAGCGAA20850.019.1262821
CTATATA12600.018.9896661
Produced by FastQC (version 0.11.5)