FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n01_2602_d10_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n01_2602_d10_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1521241
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA11400.055.3381581
GAGCAAA38150.053.7900771
GCGAAAG11650.053.52472
GAGCGAA5550.052.260951
CAAAAGC41600.049.325884
CGAAAGC12850.049.0905463
GCAAAAG42450.048.33823
AGCAAAA48300.042.3334542
ATAACGC1102.4353043E-432.9578633
AAAAGCA65700.032.115115
AAAGCAG77700.030.7946056
GCGATAA1658.411877E-526.3680231
GTGTCGA1801.5207734E-424.1667149
GTATATA10500.022.7895071
AAGCAGG110650.022.0146877
CTATGCG2256.8652787E-419.335284
TAACGCA1900.00588584319.0808684
AGTGTCG1900.005889197319.0789878
GAAAGCA34600.018.650724
GCTATTG7100.018.380328
Produced by FastQC (version 0.11.5)