FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n01_2602_d10_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n01_2602_d10_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3297100
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCAAA91100.055.0009841
CAAAAGC107150.047.303064
GCAAAAG109150.046.037713
CGCGAAA1002.733197E-643.5066262
CGAAAGC32050.039.8188633
AGCAAAA125250.039.5409552
GCGAAAG32800.038.9083672
AGCGAAA34500.036.9917031
AAAGCAG163500.035.7898566
GAGCGAA24600.034.4874841
GCGCGAA1301.6540322E-533.4671481
AAAAGCA155800.032.5787775
GCTATAT8550.027.139051
AAGCAGG212950.026.9636867
TATATCG2051.2899105E-524.759875
GTTACGT3803.6379788E-1022.8985731
CTATATA10450.022.8982282
GTATATA14100.021.0850931
TATATAC15950.020.4576643
TATTATA21400.020.33022
Produced by FastQC (version 0.11.5)