FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n01_2601_d8_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n01_2601_d8_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5749243
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA11200.060.8578381
CGCGAAA553.2177777E-452.7356072
AGCGAAA20550.048.6938321
GAGCAAA133500.043.0180361
CGAAAGC24750.041.6021733
GCGAAAG24800.041.2262762
CAAAAGC162900.035.4319344
GCGCGAA850.00274093834.1230431
TTACGTG10150.032.147942
GCAAAAG187900.029.9073433
AGTCGAA4900.029.5965162
CCGACTA13200.028.5648734
GTTACGT11500.028.3740521
TACGTGT12600.027.0476913
TCGAAGG7750.026.1974624
ACATGGT35150.025.7862135
GTCGAAG5150.025.3435923
AAAGCAG259250.024.9487636
AAGCAGG264600.024.577067
AGCAAAA226500.024.4906272
Produced by FastQC (version 0.11.5)