FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n01_2601_d8_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n01_2601_d8_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3899916
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA9050.056.8828241
CGAAAGC21950.039.6380773
GAGCAAA91300.038.8337631
AGCGAAA21000.038.669641
GCGAAAG22950.037.5952
GCTATAT8100.028.6441781
CAAAAGC129750.027.213694
ACATGGT27850.026.0339855
GTTACGT5950.025.5902041
AAGCAGG162450.024.9919857
GCAAAAG147050.023.8148563
AAAGCAG176250.023.2014876
GACGATA1902.1936109E-422.8964981
TGACGTA3803.6379788E-1022.8962045
GTATATA17900.022.6833081
GCTACTA8700.022.499719
GTCGGCT10800.022.1541966
TATAACC12750.021.6092283
AGCAAAA167100.021.0875282
AGTCGGC11650.020.5377985
Produced by FastQC (version 0.11.5)