FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n01_2601_d2_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n01_2601_d2_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3414620
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG15600.053.453382
AGCGAAA17350.047.6452941
CGAAAGC18350.045.8378073
GTATAAG11950.018.2040481
GTATATA25650.016.9620571
GAGCAAA55300.016.9152811
AGTACGG2600.001811158116.733235
ACCGTTC5201.798253E-816.7300458
AATCGCG2650.002059527416.414625145
CTACCGT3103.10297E-416.3733774
CATACCG2700.002330646216.1134835
GTCCTAT19000.015.6474971
AAAGCAG112350.015.2959765
ATACCGT3355.597602E-415.1514846
TAGTACC10600.015.0493844
CAAAAGC58500.014.9979324
TATACTG21800.014.635185
GAGCGAA10200.014.2181951
GTACGGT3700.001187763513.7182346
ATAATAC30050.013.5128043
Produced by FastQC (version 0.11.5)