FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n01_2601_d2_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n01_2601_d2_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3976060
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTAAAGAGTGCTTGCACTCAATCCTTCCAGCTTTTTGAAATTTCCCTT44600.11217134550283447No Hit
TCTTAAGACTGCTAGCAAAGTTAGTGTTGGTGGGGGAGAAGAAATGTTGG41770.10505374667384294No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA28500.065.639231
GCGAAAG30300.062.2161182
CGAAAGC33900.055.3952183
CTACGGA8000.034.4402123
TACGGAC10350.027.320994
TCTACGG10400.026.4924742
GTATACG2507.8342055E-826.1030391
CTAAGCG5700.025.4405983
TTATATC12500.023.7818742
TATACCA29350.023.468635
GTTATAT23550.023.0918621
TCTAACG2252.6524931E-522.5573333
TATACGG2903.28866E-722.5017722
AACGTGC12950.022.3933338
CTCTACG12400.022.220331
TATATAC15650.020.3849933
CTATAGA13050.020.0023291
ACGTGCC14900.019.9492269
GTAATAT23100.019.4611421
AAAGCAG133600.019.2118435
Produced by FastQC (version 0.11.5)