FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n01_2601_d12_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n01_2601_d12_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3623704
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAAAAGACTGATCAAGAGATTGAAGCAAGAAGGGGAAAAGAAACCTATA36350.1003117252402514No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA11350.068.993291
AGCGAAA22100.053.4778941
GAGCAAA93650.051.0218961
GCGAAAG25500.047.4848672
CAAAAGC103950.044.57134
CGAAAGC28050.043.1680643
GCAAAAG109550.042.0281523
AGCAAAA124300.037.099242
AAAAGCA156850.029.7238755
AAAGCAG191300.028.3129146
GTAGAAA35900.024.8421751
TTACGTG6100.023.7727342
TAATGCG2451.9128202E-623.6757034
GACCGGC6850.023.284296145
AAGCAGG251050.021.918897
ATACCGA1700.003092650821.325546
GTTACGT6500.021.1943071
CTCCGAC4151.0950316E-920.963518145
GTATATA22450.019.378221
CGAGATC6000.019.3351575
Produced by FastQC (version 0.11.5)