FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n01_2601_d12_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n01_2601_d12_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3224882
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCAAA87900.055.5971031
GAGCGAA15800.047.2673531
CAAAAGC109550.045.3369834
GCAAAAG114450.043.2058943
AGCGAAA28700.038.6536671
CGAAAGC30000.038.1865353
AGCAAAA130300.037.5607072
GCGAAAG31000.037.1886062
AAAGCAG159600.035.6624536
AAAAGCA161900.030.3638825
AAGCAGG192450.029.723037
GTTACGT3201.8189894E-1229.456051
CGGTAAC1358.0819434E-426.8545191
CTATATA9050.023.2339922
GCAGGGG95000.021.7501379
ACATGGT20050.021.6975785
GCTATAT7100.021.4457931
GTATATA12900.021.358711
TATTACG1700.00309276521.3253172
CCGAGGT10450.020.8151444
Produced by FastQC (version 0.11.5)