FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n01_2601_d10_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n01_2601_d10_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2873795
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA7000.062.1515541
GAGCAAA70350.051.329141
AGCGAAA15450.049.278741
CAAAAGC78100.046.5133824
GCGAAAG17800.042.7720952
GCAAAAG84250.042.4295273
CGAAAGC18600.040.9324343
AGCAAAA102400.034.41342
ACGCGTT1153.1641958E-431.520664145
AAAAGCA115900.031.4684815
AAAGCAG133950.030.5300566
GCCGTCG3151.8189894E-1229.919615145
CGAAAGT1804.6995083E-628.1978994
CGCAAAA1855.8155656E-627.4357952
AAGCAGG165850.025.047827
GTATATA19700.023.9246671
TATATCG2053.665715E-421.222115
GACGACT2104.313277E-420.7168244
AGTGTCG1750.00366072920.7139428
AGCAGGT38650.020.633558
Produced by FastQC (version 0.11.5)