FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n01_2601_d10_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n01_2601_d10_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4218706
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA16600.049.792451
GAGCAAA101250.047.2623831
CAAAAGC135000.035.822314
GTTACGT5750.035.306681
GCAAAAG149050.031.9104843
AGCGAAA31800.030.324331
CGAAAGC32750.029.4443423
AAAGCAG194250.029.4121486
GCGAAAG33550.028.7422432
AGCAAAA165450.028.309182
AAGCAGG208150.027.271947
ACATGGT26250.024.858495
CCGACTA9050.024.8355984
AAAAGCA196150.024.7655535
GATAGCG6550.024.350733145
CTACTGA45050.023.4973876
TACGTGT8050.023.4174183
TATACTG20950.022.495255
CGCAAAA4307.2759576E-1121.9197942
GCATGTT45500.021.8308544
Produced by FastQC (version 0.11.5)