FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n01_2599_d8_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n01_2599_d8_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2981175
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA6850.064.572331
GAGCAAA52050.047.6444741
AGCGAAA12950.047.5944481
GCGAAAG14050.045.414962
TTATCGC1602.0008883E-1145.318192
CGAAAGC15100.042.737163
CAAAAGC72950.034.5896764
TATCGCT1956.9940143E-933.465743
GCAAAAG85200.029.0206643
GGGCGAT1050.00771118227.618817
AAAGCAG118500.025.149156
CTCCGAC3551.546141E-1024.50642145
TTACGTG3851.6370905E-1124.4835952
GTTTACG2701.6486229E-724.1705151
TACCGTC1550.001808004323.3900364
GCTATAT14350.023.2441181
GCTATTG13800.022.5907658
CTAGTAC4505.456968E-1222.558393
AGACGGT7750.022.451044145
AAAAGCA113750.022.310875
Produced by FastQC (version 0.11.5)