FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n01_2599_d8_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n01_2599_d8_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3212006
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCAAA72000.052.3671651
GAGCGAA11850.048.95081
ACTCCGA6450.041.5939561
ACATGGT25400.040.8197365
AGCGAAA18900.036.4460071
TTATCGC1458.7016997E-735.002412
CAAAAGC113050.033.22214
CGAAAGC21100.032.9880523
GCGAAAG21100.032.644432
GTCGGCT9700.031.3939116
GTTACGT5850.030.9864851
GCAAAAG121650.030.7542763
AAGCAGG145400.030.3160957
GCTACTA7850.029.5534139
AAAGCAG149650.029.3605236
CAGTCGG11750.028.3849324
TACGTGT6200.028.0664483
GTTGACA37150.027.9103571
GGTCCTA43600.027.7688149
GTCGTCG2104.993308E-727.61837145
Produced by FastQC (version 0.11.5)