FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n01_2599_d6_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n01_2599_d6_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3804198
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATAACACTGTGGTTTTACACAATCTTTTAGCTAAGTCTATTGAAAGTA50740.13337896713052264No Hit
CTCTAGGATTAAACTGAAATGTTTTCTTACATTTGTCCCCACTATGTCCC44610.1172651896667839No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA7350.057.2212261
GAGCAAA71950.049.3834881
GCGAAAG14000.040.917572
AGCGAAA14300.039.55261
CAAAAGC94150.037.584634
GCAAAAG99450.035.800373
TATATCG5000.034.8058325
CGAAAGC17150.033.8249053
ATCGTGG5750.032.782078
AGACGCG3650.031.780296145
TATCGTG5500.031.6358417
GCGATTA1902.0794869E-730.525814145
GTTCGAC1050.00770482327.6236742
AAAGCAG149400.026.7431016
GGATTAA29450.026.099456
GCGCTAT6300.025.3217036
AAAAGCA147550.024.6212025
CTAGGAT43400.024.3934863
AAGCAGG166100.024.311767
GCTATTG17350.024.235918
Produced by FastQC (version 0.11.5)