FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n01_2599_d6_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n01_2599_d6_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3153751
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA9950.059.7568861
GAGCAAA63350.049.7897381
AGCGAAA18100.040.4613151
CGAAAGC20100.038.239043
GCGAAAG20100.037.8782962
CAAAAGC96150.033.031024
GCAAAAG95400.032.682653
TATTCGC1153.1611059E-431.5260331
GTCGGCT7100.027.574186
AAAGCAG136000.027.51116
TATATCG2654.5602064E-927.3622195
AAGCAGG138800.026.742927
TTATCGC2207.4856507E-726.367234
ACATGGT21000.024.5152455
AGCAAAA129100.024.1512372
GTATTAT31350.022.8978561
GGTTATA12250.022.492861
CCGACTA3955.8935257E-1022.0283164
TATATAC21400.022.0240293
GCTATAT11450.021.5313171
Produced by FastQC (version 0.11.5)