FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n01_2599_d4_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n01_2599_d4_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4134561
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTATTTTTTCATTTACCTCAAAGTATTTCTGTGTTCCTATTTTGGTT60650.14669030158219942No Hit
GTATTGGGAGTCAGGATATGGTCATTGGAGAAGAATTTATTGTGTTTATT45260.10946748639093727No Hit
ATTATATATTGCTTAATTTTTCAGATGTGCCAATGATTTTACAAAATGCT43170.10441253617977822No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA5650.062.883981
TATATCG4900.047.3517235
AGCGAAA11300.046.2004741
TTATCGC2150.043.841642
GCGAAAG12250.043.2084542
TATCGCT2300.040.9824033
GAGCAAA71100.038.5491331
CGAAAGC14550.036.8765833
TATCGTG6550.036.525557
ATCGTGG6800.035.18278
CCGACTA6250.031.3231664
CAAAAGC99300.027.4549394
TAGATGT31800.024.625134
GACATGG26600.023.9873874
ACATGGT26300.023.7096215
GGTCCTA35350.023.3797369
GCAAAAG115950.023.3249113
ATATGTC46050.022.9882075
CTTTAGT32100.022.58851
TATATTG37350.022.324885
Produced by FastQC (version 0.11.5)