FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n01_2599_d4_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n01_2599_d4_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3472323
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA10600.043.7815061
GAGCAAA77550.041.796731
AGCGAAA18650.038.492221
CGAAAGC20350.036.345123
GCGATTA2002.237357E-1036.248726145
ACATGGT24850.035.891295
GCGAAAG21650.033.8282932
CCGACTA5050.030.1535384
CAAAAGC108450.029.820554
GCTATAT7450.029.1999151
GTCGTCG2003.2592652E-728.998981145
GTCGGCT8200.028.2973986
GCAAAAG114950.027.6296563
AAGCAGG134450.027.446447
ACTCCGA6650.026.1701471
TATATCG2507.82984E-826.1043475
GGTCCTA43350.026.0898029
GTTGACA35550.025.9048271
GTAGCAT45450.025.846261
GTATATA18700.025.5928671
Produced by FastQC (version 0.11.5)