FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n01_2599_d12_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n01_2599_d12_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2473266
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA11150.051.3770331
GCGAAAG11900.048.1389852
CGAAAGC14200.041.3631363
TCGAATA2558.913048E-1131.273859145
GTATAAT18150.023.9712891
TATATCG2202.227397E-523.0723675
ACCGTGC4952.1827873E-1120.5046238
TATAATA23750.019.5403862
TATATAC15350.019.3683343
GTATATA14250.019.3368421
GTACCGT3605.2935204E-516.1140356
GAGCGAA5854.3764885E-916.1140351
TACCGTG4109.037869E-615.9143047
CAAAAGC38850.015.6785194
AAAGCAG82500.015.6452635
CTAGACG3355.5953715E-415.1520023
TATACTG16900.015.0175165
TTATATC13700.014.8202074
TATATAG14300.014.7054642
GTATAAG9900.014.6491221
Produced by FastQC (version 0.11.5)