FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n01_2599_d12_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n01_2599_d12_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4254829
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG21550.058.209042
CGAAAGC24050.053.9671633
AGCGAAA24950.050.277341
TACGAAC3952.3646862E-1123.860629
TATATAC23200.022.1902373
GTATATA25350.020.8805351
TATACCA33400.018.8872815
GTATTAA34800.018.7525371
GTATAAA21700.018.0439131
CTATATA17250.017.6543542
TATACGA5451.715307E-917.2934767
CGCCTAT5552.1791493E-916.984281
TATAATA23350.016.7686632
ATATACC24000.016.6168294
GTATAAG11350.016.6101761
GAGCGAA16350.016.4089451
TTTATAC26000.016.1750743
GTATAAT18850.016.156031
TATTATA22900.016.1482812
TAGTACC15300.016.1132894
Produced by FastQC (version 0.11.5)