FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n01_2599_d10_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n01_2599_d10_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2594108
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACATAATGGCATACAAAAGACTGATCAAGAGATTGAAGCAAGAAGGGGAA28180.10863078946597444No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA6000.064.047681
AGCGAAA13000.052.985751
GAGCAAA53150.048.428821
GCGAAAG14800.046.542432
CGAAAGC16650.041.371053
CAAAAGC68050.038.038844
GCAAAAG72250.035.727233
AGTGTCG1652.3873345E-630.7592818
CTCCGAC2651.382432E-1030.093107145
GGCATAC18750.028.6149188
AAAGCAG107900.027.620046
CTATATC18400.026.0083794
GGTTATA8650.025.1468721
TATCGTG2051.2911278E-524.7565177
GCTATAT8800.024.7182331
CATAATG23800.024.3725092
AGCAAAA106850.023.9545142
ACATGGT12200.023.7731845
CAACGTC2451.9134386E-623.6743269
ACATAAT24150.023.4183481
Produced by FastQC (version 0.11.5)