FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n01_2599_d10_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n01_2599_d10_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3339461
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCAAA79350.054.0981061
GAGCGAA15000.050.75811
CAAAAGC113600.037.787154
GCAAAAG116200.036.567253
GCGAAAG25600.035.6887552
TATATCG2252.0008883E-1135.449575
AGCGAAA25050.035.0255471
CGAAAGC25950.034.6485563
GTTACGT4700.033.9415861
TTATCGC2455.4569682E-1132.555732
AAAGCAG157300.031.7146936
AGCAAAA141450.030.0909522
GCTATAT8850.028.6768931
ACATGGT21050.028.2463655
AAGCAGG178300.027.974767
AAAAGCA168500.025.1742615
GCCGTCG2901.19307515E-824.999496145
TATCGCT3251.4861143E-924.542013
ACTCCGA7400.024.4971521
GTCGTCG2401.6002323E-624.16618145
Produced by FastQC (version 0.11.5)