FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n01_2597_d8_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n01_2597_d8_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3118708
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACATAATGGCATACAAAAGACTGATCAAGAGATTGAAGCAAGAAGGGGAA33690.10802550286849555No Hit
ACAAAAGACTGATCAAGAGATTGAAGCAAGAAGGGGAAAAGAAACCTATA33070.10603750014429052No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA9850.054.472821
GAGCAAA69250.051.4098171
AGCGAAA18150.049.536971
GCGAAAG19950.046.1578062
CAAAAGC77100.045.9888734
CGAAAGC22050.041.7618263
GCAAAAG84300.041.2855453
AGCAAAA104900.032.970632
AAAAGCA119900.029.632975
AAAGCAG144400.028.2206756
TATATCG1958.752828E-626.0292535
GTATATA21400.023.039841
GGATCGC1600.00217644922.655762145
GCTATAT16550.021.905661
AAGCAGG189150.021.88637
TAGATGT27700.021.7268414
CTAGTAC4501.3278623E-1020.9466783
GTATTAT30350.020.3069461
TCTCCGC11450.020.261572145
TATACTG13450.019.9470065
Produced by FastQC (version 0.11.5)