FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n01_2597_d8_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n01_2597_d8_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3272836
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA8750.051.377811
GAGCAAA70250.045.9309771
AGCGAAA18550.036.7431071
GCGAAAG20350.033.8494152
CGAAAGC21000.032.8016933
CAAAAGC102200.032.3523564
GCAAAAG106500.030.3652743
AGCAAAA121850.026.004462
AAAGCAG145350.025.64136
GTATACG1450.001226151225.0031051
AAGCAGG154400.024.3228637
GTTACGT4900.023.6764091
GCTATAT9100.023.1072671
ACATGGT22100.022.9666545
GTACTAG3905.0386006E-1022.3104631
AAAAGCA148050.022.0392115
CTATATA14600.020.8587572
CGAAGCG2104.3174054E-420.7140129
CTAGTAC5403.6379788E-1220.1413923
TAGTACT9900.019.7751834
Produced by FastQC (version 0.11.5)