FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n01_2597_d6_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n01_2597_d6_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGCAGGTCAGCCTGCAGTGTCCCGGCAAGGCCCTGTGTGTCTTTGAAGC11.8867924528301887No Hit
GTCGCTTACAATCTGTTCACCAATTATTCAAATAACAACATAATTTCATC11.8867924528301887No Hit
TTGGGGATTCATTTTCCAGAGTGTCTCAGTGTACACTTAAAACAATCAGT11.8867924528301887No Hit
GTCTTCAAATCTGAAATCATATAGATTATAGCCTTTCAACCCTGTAACAT11.8867924528301887No Hit
GCAGAATACTAAGATCACAAAAGACTGGTGAAACAAAAGCGACAAAATTA11.8867924528301887No Hit
CCTCATAATTATGTCCTTGAACACAAGCTAATGGACAAATTTCTCCAACA11.8867924528301887No Hit
CTCCAACACCATTTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC11.8867924528301887No Hit
TGAAAAAGGACTGCAACACCATGTCAACGTTTAAGGTACACTGTATCCTT11.8867924528301887No Hit
TAACTACCTACAGACCAGAAATGAAAAATGGCGAGAGCAATAGGTACAGA11.8867924528301887No Hit
TAATAGCAGACCTTCACAACCTCCAGACCATAAATGAAAAAATGAAAGAG11.8867924528301887No Hit
TATATCGATCTTTCAATCAGTCCACCAAAAATACCAGATACAGCAGAGAC11.8867924528301887No Hit
TTCCTTGATCGGCTCCGCCGAGATCAAAAGTCCTTAAAAGGAAGATGCAA11.8867924528301887No Hit
AACGCAAAAAACATTTAAACAGCCCCAGGCAAATCGCACGTACAAGATAA11.8867924528301887No Hit
AGTATGAACTGGAAGAGAAGCAAAACGGCAAAATACTCAACCTATTGCAA11.8867924528301887No Hit
AGATACGGACCAGCATTAAGAATCAATGCACTGAGTAACCTTGCTAAGGG11.8867924528301887No Hit
CTGCAGGTTAGGCGCCGCTCGGGCGCCGGCACGGAATAGTGGCGGTTGAA11.8867924528301887No Hit
TCTAATTTTGTGACCTCAACATATTTCTACCGGTACTGAATGCTCCCGTC11.8867924528301887No Hit
AGCGAAAGCAGGATGCAGGGGAAGAAGGGGTAGAGAGAGAGGGAGAAAGA11.8867924528301887No Hit
ATAAAACTATCATACAATAATATACCAAAATCAAAATAAAAATACATCAA11.8867924528301887No Hit
TAGTAGGACTGGAAAAGAATGGAAACGCTGTTCAAGACTCTGAAGATAAA11.8867924528301887No Hit
AAACAATTCTATGACGATTTCAATCTGTCCCGCATTTCATCAATTAACCA11.8867924528301887No Hit
CCCTCGAGGAAAATTCAAGCGAATGGTTCAGTCTCATTACAAGGAAAAAG11.8867924528301887No Hit
ACCATGAACCAGTATCGTGACATTTCCTAGATTGTCAAGTCAGAAAGGTA11.8867924528301887No Hit
CTACTGAACAACCAATTGGGTTCATGGGCAAACCAAAATAGGAACACCGA11.8867924528301887No Hit
ATTGAATGCTTTTAAGACAAGCTGCATGTCCTCTTTCATCACTATTTTGA11.8867924528301887No Hit
TACCAAAAAATAACAATTAACCAAGCCTTACAACTAAAGCATGAAGTAGA11.8867924528301887No Hit
GCAACATTCTTAAAGGAAAATTTCAAACAGCTGCACAAAGAGCAATGGTG11.8867924528301887No Hit
CCTGGTACCTCAGCATGTTTCTGCATTTGGAGACAGGGCTTTTAAAGAGG11.8867924528301887No Hit
CTCCTCAGTGAAAGCCCTTAGTAGTATAAAGCTCTCTAATCGGTTAAAGA11.8867924528301887No Hit
ATTATATTCACCATGGAAAGAATAACAGAACCACGGTTACTAAATTCGCA11.8867924528301887No Hit
ACTAAGGGCTATCAATGAGGAGGCATAAAAACTTCCAGAAATTTCACCAT11.8867924528301887No Hit
CTGGAAAGCATATTGAAAGATGAAACCACAGAAACCCATAGAATGACAAT11.8867924528301887No Hit
ACAAAGTAATATTGAATAATAAATCAAATCAAATAAGAAAAGACGAAATA11.8867924528301887No Hit
CATGAATCGAAATGTAACAGGGCTCGAGACGAATTATATAAGCTGCGATT11.8867924528301887No Hit
ATACCAGGGAAGGCAGAGAGGGTAGTAGACTCCCATCTGTGTGGTCCTGC11.8867924528301887No Hit
CAATTAAACACCATATTTCCTCAAATTTCTGTCCCAATTGCACTCGCAAA11.8867924528301887No Hit
ATAGGAGATAAAGGGAGGCATTTCCATGGGCCTCCAGGACCGCTAGGGGC11.8867924528301887No Hit
TAACATATCAACAAAGACCTGGTTCTTTGAAAAGAACAAAGATGACAGAG11.8867924528301887No Hit
CAGTAGATACAGAGAAATAAAGATAAAAATTACACTGGCCATTCACCAGA11.8867924528301887No Hit
GAGCAAAAGCTGGCCGACCAGCACACTGAGCGAAACTAGAATCAGTCTCA11.8867924528301887No Hit
CACTTAGAATGACAATTCCATCTGAACAAAAATCGCGCAACCTTACACAC11.8867924528301887No Hit
CCGCTGGGTTTGCTACTGCGGGTCACTCCACCCACAAAGCCGGGTGCTGC11.8867924528301887No Hit
AGTTGTAAGGCTACCATAAATGTGATTTGTTCTAAACTATTCTCTGTCGC11.8867924528301887No Hit
ACTTTACATATAATGATGAACCCACTCATTAATGCCGCATAAACAACTGA11.8867924528301887No Hit
CATTTGAAATTATTTGTTCTTTTATGTTTAATTGAGGTAGCATATTAAGT11.8867924528301887No Hit
CCATTATATAGATCGTGATTTTTCATACCTATTAGAAAAGGGGTGATTAT11.8867924528301887No Hit
CATTAGAGAAGATTCCTACGTGCCCCCAACCCCCGCATAGCACTTTAGCC11.8867924528301887No Hit
GAAAATGTAGGGTTCACAGTGGTGTGAGCACAGGTGGGGCTAGGTTCAGT11.8867924528301887No Hit
AAACAAGGGAGCAGAGAATATATTCTGTTGAATTAACAGCGAGCAAATTT11.8867924528301887No Hit
ATATAGATGTTACATTCATGAAATAAGATAATGGCATACAAAAGCCTGAT11.8867924528301887No Hit
CTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACTA11.8867924528301887No Hit
CGAAACTATGAGATCAATCAAACTTGAACTTATACTCTTCTAATATGCTT11.8867924528301887No Hit
GCTCACATGTTGCACACACCAGGCCAAATTCCACTTAAGTGGTTTCAGCC11.8867924528301887No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGAAGA50.0145.06
AGAAGAT50.0145.07
ATTAGAG50.0145.02
GAAGATT50.0145.08
CATTAGA50.0145.01
AAGATTC50.0145.09
AGAGAAG50.0145.05
ATGTCAA50.0145.0145
TAGAGAA50.0145.04
TTAGAGA50.0145.03
Produced by FastQC (version 0.11.5)