FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n01_2597_d4_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n01_2597_d4_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2858183
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTCAATTATATTCAGCATGGAAAGAATAAAAGAACTTC241220.8439627553589115No Hit
ATTATATTCAGCATGGAAAGAATAAAAGAACTTCGGAATCTAATGTCGCA175320.6133966929339374No Hit
CAATTCTTGGAAAAGATGCCGGCACTTTAATTGAAGACCCAGATGAAAGC138950.4861480178141147No Hit
GAGCAAAAGCAGGTACTGATTCAGAATGGAAGATTTTGTGCGACAATGCT132120.46225171726233066No Hit
CATTAGCCTTTTCCCCCTTAGCAAGGTTACTCAGTTCATTGATGCTTAAT129960.45469446847875034No Hit
GTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG125790.44010477985489377No Hit
GCAAAAGCAGGTCAATTATATTCAGCATGGAAAGAATAAAAGAACTTCGG105970.3707600248129668No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA86820.30375941638446524No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG83730.2929483521523989No Hit
TTTTTAAACTATTCAGTATTAATTGATGGCCATCCGAATTCTTTTGGTCG75710.2648885673170682No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGACAGACGCATTTGAAAAAAAGACG75490.2641188475335554No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCATGGGGCCAAAGAAATAG74820.26177470092013005No Hit
GCTATAACTATTTTAATGCATGTGTCAGGAAGGAGTTGAACCAAGACGCA74580.26093500661084335No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC74350.26013029956444356No Hit
AAAGAGTATGGGGAGGATCTGAAAATTGAAACTAACAAATTTGCAGCAAT73310.25649162422420113No Hit
AGCAGGTACTGATTCAGAATGGAAGATTTTGTGCGACAATGCTTCAATCC64750.2265425271929754No Hit
GTGTTACTGTTTACTACTGTCCAGGCCATTGTTCTGTCTCTCCCCTCGAT63560.2223790429094288No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT60650.21219774940932756No Hit
TAATTAAGAAGTACACATCGGGGAGACAGGAAAAGAACCCGTCACTTAGG58280.20390576810512132No Hit
ATTCAGTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT57810.20226136674943487No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC55210.1931646783988289No Hit
AGCTAATAGAGTCCTACAGACTTTTCCAATGGAACCTTCTTCCACTCCTT54670.1912753662029338No Hit
GAATAAAAGAACTTCGGAATCTAATGTCGCAGTCTCGCACTCGCGAGATA54170.18952600305858652No Hit
GTACGGATAACAAATAGCAGCACTGCTATAACTATTTTAATGCATGTGTC53760.18809152528022174No Hit
CTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACCTCTGACTAAGGG53390.18679699655340473No Hit
GAAAGAGTATGGGGAGGATCTGAAAATTGAAACTAACAAATTTGCAGCAA51630.18063923828530223No Hit
CCTTAAGGTAATATATGTGGACTTCTCTCCTTGTCACTCCAATTTCGATG51040.17857498977497244No Hit
GCAAAAGCAGGTACTGATTCAGAATGGAAGATTTTGTGCGACAATGCTTC50270.17588097053267757No Hit
TCTCAGGACGGCGGACTCCACTCCGGATGTGCTTTCATCTGGGTCTTCAA49900.17458644180586058No Hit
GTATGGGGAGGATCTGAAAATTGAAACTAACAAATTTGCAGCAATATGCA49620.17360679844502608No Hit
ATATACAATAGAATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT49610.17357181118213913No Hit
CAATAGAATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGTGTG49600.1735368239192522No Hit
CAGTATCTGCAACACTACTGGAGCTGGAAAACCGAAGTTTCTACCGGATT48450.1695132886872534No Hit
CAATTATATTCAGCATGGAAAGAATAAAAGAACTTCGGAATCTAATGTCG48440.16947830142436646No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA46250.16181609085212528No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC45400.15884217350673488No Hit
GAATAACTGAGAGCTATTTCTTTGGCCCCATGGAACGTTATCTCCCTCTT44850.15691787404795285No Hit
GTTAAAGCACAGATTTGAAATAATCGAGGGGAGAGACAGAACAATGGCCT43260.15135489924892842No Hit
CCATATGGCCATAATTAAGAAGTACACATCGGGGAGACAGGAAAAGAACC42050.14712144043960795No Hit
AGCAAAAGCAGGTCAATTATATTCAGCATGGAAAGAATAAAAGAACTTCG41910.1466316187591907No Hit
ATTAAGAAGTACACATCGGGGAGACAGGAAAAGAACCCGTCACTTAGGAT40430.1414535038519227No Hit
CCTTTGGGGGACTCCCCAATGGGCCATGCTTCTGATTTATTCTCAAAAAA40340.1411386184859402No Hit
AGCGAAAGCAGGTCAATTATATTCAGCATGGAAAGAATAAAAGAACTTCG39880.13952920439314068No Hit
ATTCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATA39840.13938925534159288No Hit
CAGTTATTCTGCTGGTGCACTTGCCAGTTGCATGGGCCTCATATACAATA38040.13309154802194262No Hit
ACAATTCTTGGAAAAGATGCCGGCACTTTAATTGAAGACCCAGATGAAAG37710.1319369683466734No Hit
GTCTCGCACTCGCGAGATACTGACAAAAACCACAGTGGACCATATGGCCA36370.12724867511982263No Hit
CTCATAGACTCAGGTACTCCTTCCGTAGAAGGGCCTCTTTTAAGGCCGTG35690.1248695412435103No Hit
TCATATTCAGATTTCCATTTCATCAATGAACAAGGCGAATCAATAGTAGT35240.12329511441359774No Hit
GTATCTGCAACACTACTGGAGCTGGAAAACCGAAGTTTCTACCGGATTTG35050.12263035641874576No Hit
GCCTTAGCTGTAGTGCTGGCCAAAACCATTCTGTTCTCATGTTTTATTAA34790.12172068758368516No Hit
CCGTATCTTCTGTCTTCTTTACCTATAATGAGGAACCCTCTCAGGACGGC34060.1191666173929381No Hit
GGCCTGGTGTGTGCAACATGTGAGCAGATTGCTGATTCCCAGCACAGGTC33970.11885173202695559No Hit
GTACACATCGGGGAGACAGGAAAAGAACCCGTCACTTAGGATGAAATGGA33740.11804702498055583No Hit
CATTTGGAGGTGTGTTTCATGTATTCAGATTTCCATTTCATCAATGAACA33670.1178021141403472No Hit
TCCTAGTTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGA33620.11762717782591246No Hit
CAGCTATTCTGCTGGTGCACTTGCCAGTTGCATGGGCCTCATATACAATA33500.11720733067126914No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT33440.11699740709394744No Hit
GACTTGAAGATGTCTTTGCTGGGAAAAACACAGATCTTGAGGCTCTCATG33230.11626267457332158No Hit
GTGCTGGGAATCAGCAATCTGCTCACATGTTGCACACACCAGGCCAAATG33180.11608773825888687No Hit
ATCTAATGTCGCAGTCTCGCACTCGCGAGATACTGACAAAAACCACAGTG32900.11510809489805236No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGAGAG32710.1144433369032004No Hit
GTAGTAAACAGTAACACTACTGGAGCTGGAAAACCGAAGTTTCTACCGGA32120.11237908839287059No Hit
ATTAACCATTTCTGTTATCCTTTTGTCAGCAGTGATTGGATATTTCATTG32040.11209919028977501No Hit
ATTGAATACTGACTTAGCTAATAGAGTCCTACAGACTTTTCCAATGGAAC31960.11181929218667944No Hit
ATACAATAGAATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGT31390.10982501820212351No Hit
GTATACTAGAGTCCCGTTTTCGTTTCATTACCAACACCACGTCTCCTTGC31120.10888036210417598No Hit
GGTCAATTATATTCAGCATGGAAAGAATAAAAGAACTTCGGAATCTAATG31060.10867043852685429No Hit
ATATTCAGCATGGAAAGAATAAAAGAACTTCGGAATCTAATGTCGCAGTC30860.10797069326911539No Hit
GAATAAGGGATATCCCTAGCAGAATAAGCATCTATTGGACAATAGTAAAA30810.10779575695468065No Hit
ACCTTAAGGAGTGCTATAGGCCAAATTTCAAGGCCGATGTTCTTGTATGT30560.106921075382507No Hit
CACCAGGCCAAATGCCACTTCAGTGGTTACAGCCCCCATTCTATTGTATA30250.10583647023301168No Hit
GAGCAAAAGCAGGAGTAAAGATGAATCCAAATCAAAAGATAATAACGATT29930.10471687782062938No Hit
GTTCTGTCTCTCCCCTCGATTATTTCAAATCTGTGCTTTAACAGTGCATT29520.10328240004226462No Hit
GGTTCGTCCCTAAGAGCCTGAACAATAAGGAGCAGTTTTCTTGACTCTGC29250.10233774394431706No Hit
GGTATGGGGAGGATCTGAAAATTGAAACTAACAAATTTGCAGCAATATGC29240.10230275668143013No Hit
GCTAAAGACAAGACCAATTCTGTCACCTCTGACTAAGGGGATTTTAGGGT29160.10202285857833457No Hit
GAGCGAAAGCAGGTCAATTATATTCAGCATGGAAAGAATAAAAGAACTTC29050.10163799868657816No Hit
GCTTAATGCTGGTCCGTATCTTCTGTCTTCTTTACCTATAATGAGGAACC28890.10107820248038701No Hit
GGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATCAGAAACGAAT28750.10058838079996978No Hit
CCCCAATGGGCCATGCTTCTGATTTATTCTCAAAAAACTCTTTGGTCATG28730.10051840627419589No Hit
CTCTTAGACCAGTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCC28730.10051840627419589No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA10450.0102.701441
GCAAAAG112800.079.008523
CAAAAGC116700.076.243854
AAGCAGG131850.075.934367
AGCAGGT116600.073.616968
CTCCGAC851.6916601E-1068.23321145
AGCAAAA133300.067.347522
AGGCTCG2300.066.193634145
GCGCGAA451.1929702E-464.458271
AGCGAAA18500.063.108121
GCAGGTC75550.060.358859
TTTAAAC21500.057.0005953
ACTATTC22100.055.767538
CGAAAGC21200.055.754883
TAAACTA22750.055.1436925
GCAGGTA73700.054.693049
GCGAAAG22300.053.329822
CTATTCA23300.052.8953829
GCAGGGG8000.052.5608989
ACGGATA19650.052.403093
Produced by FastQC (version 0.11.5)