FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n01_2597_d12_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n01_2597_d12_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3316012
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA16300.055.6005551
GCGAAAG19350.047.5860372
GAGCGAA8800.046.9622541
CGAAAGC20600.044.698533
GAGCAAA65250.043.0018581
CAAAAGC74850.036.033624
GCAAAAG89700.030.3914993
TAGTGCG2156.1327046E-726.976289
ATCGTAC2351.331493E-624.6819153
AGCAAAA110750.024.2222652
AAAAGCA115650.023.5721385
AAAGCAG143650.022.40966
CTATTCG2003.1054963E-421.7496269
GCTATAT13900.020.3426061
AAGCAGG163100.020.224677
CTCCGAC4702.382876E-1020.052546145
CTGATCG8150.019.5702179
TCGTACA3001.1063761E-519.3341674
GTATAAG8450.018.8765541
TCTCCGC11700.018.589144145
Produced by FastQC (version 0.11.5)