FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n01_2597_d12_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n01_2597_d12_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences440278
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCGAC100.0070904368144.993767
CACGAGC901.3222652E-648.33125145
AGCGAAA1755.2750693E-1141.43621
CGAAAGC1951.6916601E-1037.1863333
GCGAAAG2002.2191671E-1036.2566762
CAATGCG900.00362277432.2281534
TAAGACG1000.00606535529.005344
AAGTGCG1050.007705461227.6178597
ATAAGAC2156.1016726E-726.981713
GAGCGAA1500.001491347224.1711161
GAGCAAA8250.023.731641
TCTCCGC2202.2258788E-523.067188145
GTCCTAT3503.4797267E-922.789911
GTAATAT2353.6978556E-521.5997181
TACTATA2053.657391E-421.2234172
ATATTGC2755.191456E-621.0947916
CAAAAGC9050.020.8325634
TCCTATG3901.2121745E-820.4524822
ACTGGGC5700.020.3499988
CTATATA2155.043692E-420.2362821
Produced by FastQC (version 0.11.5)