FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n01_2597_d10_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n01_2597_d10_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences768373
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACATTCTACTGAACAACCAATTGGGTTCGTGGGCAAACCAAAATAGGAAC16670.21695192308943703No Hit
GTTATGTGTTGGCCTTTGGCTCAAGTCATGAATTTAGGGTCCTGGAATTG15700.20432784598105347No Hit
ACACCTAACAGACCAAGCCACACAGGCAGCCCTTAGAGCCAACTTTAAAA14580.18975159200023947No Hit
CATCACCCTCAGACAACCCCTTACTTGGATGGACTTGACAGATACACACA12960.16866808177799064No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG11550.15031761917714442No Hit
GCAGTGCAGCAACTAAATATGTAGAGCATATATGAACAAACCTAAGAGAA9840.12806280283143734No Hit
CCACCTTAGGTCATATGTAAGGCCACAAGACAAGTCAATAAATTCCAGAA8630.11231524272716506No Hit
CTCCACCTTAGGTCATATGTAAGGCCACAAGACAAGTCAATAAATTCCAG8610.11205495247750767No Hit
ACATTCTACTGAACAACCAATTGGGTTCATGGGCAAACCAAAATAGGAAC8520.11088364635404939No Hit
CCTCAGAGTATATTATGAATATTTGCTTTTAGAATAGCTTCTATTTAAAT8310.10815059873264678No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCCGTA259.381865E-487.0094452
TTACGTG2500.075.408192
GTTACGT2550.073.9295961
ACGTGTT2600.072.507874
TACGTGT2750.068.55293
ACGTCCG350.003566493362.1415189
GATATAC2500.058.00633
GCGACTA502.008871E-458.0062984
GTAGCAT9800.054.7508431
GCCGTAG400.006047755554.3809053
CATGTTG7350.054.2575955
CTCGACT553.2188738E-452.7261358
GTGTTGG11200.051.143956
ACATGGT5550.050.951485
GTTATGT7150.050.7048071
GTAATGC1152.4811015E-950.4402581
GCATGTT10100.050.252984
TAGCATG10250.049.5175742
CGTGATA450.00963911448.332298
TGTTGGC12400.047.357857
Produced by FastQC (version 0.11.5)