FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n01_2597_d10_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n01_2597_d10_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3402371
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC37590.11048177873606375No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA8500.040.948881
GTTACGT6250.040.607641
TACGTGT6850.038.108243
ACGTGTT7950.032.83544
AGCGAAA16800.032.803791
GCGAAAG17650.032.4555782
CGAAAGC17100.032.2273373
GAGCAAA74900.031.94861
TTACGTG8450.030.8924792
AGTCGAA3750.027.0709632
ACTCCGA9950.026.9648721
AGTCGGC8550.025.4426355
ACATGGT30550.025.1594755
AAGTCGA4500.024.1712151
GCTACTA6950.023.9923829
GTCGAAG4303.6379788E-1223.6083983
GTCGGCT9250.023.5172466
GTAGCAT35600.023.016971
AAGCAGG119800.022.8755597
TAGTACC19500.022.6790879
Produced by FastQC (version 0.11.5)