FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n01_2595_d8_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n01_2595_d8_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3389036
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA11000.067.89361
AGCGAAA21200.051.3026351
GAGCAAA88200.049.3249861
CAAAAGC94050.045.409554
GCGAAAG25550.043.41952
GCAAAAG105050.040.5159763
CGAAAGC28050.040.0666583
AGCAAAA118050.036.1156652
TAGCGCG2507.094059E-1131.89916145
AAAAGCA140950.030.2484365
AAAGCAG171450.029.0542686
TAATGCG2104.987087E-727.6223984
GCTATAT12650.027.5128141
TATATAC21800.025.943133
CTATATA17450.024.5155072
GTTACGT4600.023.6438241
GTATATA18650.022.5493181
AAGCAGG230900.021.7594917
TACCATG20550.021.1679537
GTAGAAA30250.020.1343771
Produced by FastQC (version 0.11.5)