FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n01_2595_d8_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n01_2595_d8_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2981007
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCAAA69250.045.756691
GAGCGAA10800.045.653931
GCGAAAG19800.040.647722
CGAAAGC20200.040.201763
AGCGAAA20000.039.51751
CAAAAGC89800.035.526694
TATTCGC1652.3861667E-630.761471
GCAAAAG107050.029.7341983
ACATGGT22000.028.3435465
GTTACGT4400.028.014911
GCTATAT7850.027.7105141
AAAGCAG135950.027.2000276
AGACGAC4450.026.0698413
AGCAAAA119850.025.9536022
AAGCAGG141900.025.6481487
TTCGCAC2151.8660026E-523.6068443
GTTGACA26800.023.5384251
AAAAGCA138650.023.1665955
CCGACTA5550.022.2092744
GTAGCAT35800.021.4691981
Produced by FastQC (version 0.11.5)