FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n01_2595_d6_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n01_2595_d6_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3120754
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA7050.074.049941
AGCGAAA13650.044.088651
GCGAAAG14950.041.224852
GAGCAAA74250.040.818891
TTACGTG5700.040.7058142
TAGCGCG1807.2759576E-1140.27728145
CAAAAGC78450.038.541134
CGAAAGC16850.037.0066573
GTTACGT6350.036.539081
TACGTGT7300.033.7704933
GCAAAAG89750.033.1230853
GTCCGAT1102.435901E-432.9578321
ACGGTAT1504.398667E-529.0028936
AGCAAAA105600.028.1514842
GCTATTG15850.027.9014198
GAGCTAT16550.027.6009396
AAAAGCA119400.025.6872395
AAAGCAG143500.025.36496
GACGACT3201.2423698E-924.924364
AGACGAC3601.8553692E-1024.1690793
Produced by FastQC (version 0.11.5)