FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n01_2595_d6_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n01_2595_d6_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3496580
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA9400.060.1692281
GAGCAAA77250.043.7416421
AGCGAAA20650.036.5191761
GCGAAAG21850.035.509642
CGAAAGC22100.035.4360583
CAAAAGC107800.031.4131894
GTTACGT5550.028.7433511
GCGACTA1804.6979803E-628.1993734
GCAAAAG127500.026.3889263
CGCGTTG1100.00967534826.363342145
TATAGCG1701.0307893E-425.5927072
AAAGCAG159000.025.174216
AAGCAGG158000.024.9617467
ACATGGT28050.024.0416345
ACTCCGA9050.023.2357711
AGCAAAA145050.023.0960732
GTAGCAT37650.022.726091
GCTATAT8700.021.6701451
AAAAGCA159250.021.3098455
TACGTGT7550.021.129523
Produced by FastQC (version 0.11.5)