FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n01_2595_d4_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n01_2595_d4_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3936425
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTATATTCAGCATGGAAAGAATAAAAGAACTTCGGAATCTAATGTCGCA229200.5822542027347148No Hit
GTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG173990.4420000381056415No Hit
GAGCAAAAGCAGGTCAATTATATTCAGCATGGAAAGAATAAAAGAACTTC136940.3478791035012734No Hit
TTTTTAAACTATTCAGTATTAATTGATGGCCATCCGAATTCTTTTGGTCG135420.34401773182519674No Hit
GCAAAAGCAGGTCAATTATATTCAGCATGGAAAGAATAAAAGAACTTCGG95130.24166597864813885No Hit
TAATTAAGAAGTACACATCGGGGAGACAGGAAAAGAACCCGTCACTTAGG89140.22644912579307366No Hit
CAATTATATTCAGCATGGAAAGAATAAAAGAACTTCGGAATCTAATGTCG80980.20571965679518855No Hit
GAATAAAAGAACTTCGGAATCTAATGTCGCAGTCTCGCACTCGCGAGATA75760.19245889353918846No Hit
ATTCAGTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT66410.16870637697911176No Hit
ATAATGAGGAACCCTCTCAGGACGGCGGACTCCACTCCGGATGTGCTTTC64540.1639558736670964No Hit
GCTATAACTATTTTAATGCATGTGTCAGGAAGGAGTTGAACCAAGACGCA64240.1631937608362918No Hit
ATTAAGAAGTACACATCGGGGAGACAGGAAAAGAACCCGTCACTTAGGAT61540.1563347453590504No Hit
TCTCAGGACGGCGGACTCCACTCCGGATGTGCTTTCATCTGGGTCTTCAA59590.1513810119588205No Hit
CCTATAATGAGGAACCCTCTCAGGACGGCGGACTCCACTCCGGATGTGCT57350.14569056948881282No Hit
GCTTAATGCTGGTCCGTCTTCTGTCTTCTTTACCTATAATGAGGAACCCT55230.14030497215112697No Hit
CCATATGGCCATAATTAAGAAGTACACATCGGGGAGACAGGAAAAGAACC52570.13354757171799286No Hit
TAACAAGACCACTAAAAGGCTAACAATTCTTGGAAAAGATGCCGGCACTT52150.13248061375486642No Hit
GTTCATTGATGCTTAATGCTGGTCCGTCTTCTGTCTTCTTTACCTATAAT50360.12793334053106561No Hit
ATATTCAGCATGGAAAGAATAAAAGAACTTCGGAATCTAATGTCGCAGTC49900.1267647675238319No Hit
CTAAAAGGCTAACAATTCTTGGAAAAGATGCCGGCACTTTAATTGAAGAC49190.12496110049092768No Hit
ATCTAATGTCGCAGTCTCGCACTCGCGAGATACTGACAAAAACCACAGTG45070.11449475094787782No Hit
GGTCAATTATATTCAGCATGGAAAGAATAAAAGAACTTCGGAATCTAATG43350.11012530405126479No Hit
GTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTGATGGCCATCC41700.10593368348183949No Hit
CTTTTCCCCCTTAGCAAGGTTACTCAGTTCATTGATGCTTAATGCTGGTC40140.10197069676165557No Hit
AGCGAAAGCAGGTCAATTATATTCAGCATGGAAAGAATAAAAGAACTTCG39790.10108156512571687No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA10250.085.597131
AGCGAAA21650.065.3092041
GCGAAAG26750.053.3971522
CGAAAGC27300.052.055793
AAGGTCG14550.049.8274359
CTATTCA47300.046.2889489
AGCAGGT94450.044.9040378
GAGCAAA142700.044.1563871
GCAGGTC65450.043.864899
CAAAAGC142500.043.707294
GCGCGAA1053.8233775E-641.4342651
ACTATTC54900.040.9374548
CGCGAAA907.482631E-540.281272
GCAAAAG159800.038.7486653
GTAGAAA34950.035.4769821
AAAGCAG203450.035.282446
AAGCAGG206100.035.247377
ATTCAGC105950.035.1757436
GGTCGTT15300.035.065287
AGCAAAA181550.034.3860662
Produced by FastQC (version 0.11.5)