FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n01_2595_d4_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n01_2595_d4_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences243368
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTATATTCAGCATGGAAAGAATAAAAGAACTTCGGAATCTAATGTCGCA4620.1898359685743401No Hit
GTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG3010.12368100982873673No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGGTCG300.001934052872.499289
TACGGAT300.001934052872.499282
GAGCGAA1102.5465852E-1159.3175931
ATTGGCG400.006045643754.374466145
CGGATAA450.0096308248.332854
GAGCAAA6050.037.148391
GCGAAAG2002.2191671E-1036.249642
AGCGAAA1803.1795935E-936.2496381
CGAAAGC2052.8921932E-1035.36553
CAAAAGC6450.034.8446164
TTAGAGT850.00273806334.117314
AGCAGGT3400.031.9849768
GCAAAAG7050.031.8791163
GTATTAA4200.031.0711211
TTGATGG3151.8189894E-1229.9203388
TTGATTG1000.00606584528.999712145
TTATGGG1000.00606584528.9997124
TATTAAT4100.028.2924022
CAATAGG1050.00769755527.6187741
TGATGGC3453.6379788E-1227.318579
Produced by FastQC (version 0.11.5)