FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n01_2595_d2_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n01_2595_d2_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3023465
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTATATTCAGCATGGAAAGAATAAAAGAACTTCGGAATCTAATGTCGCA66140.21875563302369963No Hit
GTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG54420.17999216131160772No Hit
GCTATAACTATTTTAATGCATGTGTCAGGAAGGAGTTGAACCAAGACGCA48690.16104039570492795No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGACAGACGCATTTGAAAAAAAGACG30720.10160527738869146No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA8700.059.1785051
AGCGAAA17200.056.9154551
CGAAAGC19200.052.118943
GCGAAAG20600.048.225672
ATTATAT32950.039.393311
GTACCGA951.02827275E-438.1648986
GCGTTAA2600.036.2494549
TTATATT39200.034.7773672
GCTATAA27200.034.1244741
CAAAAGC96900.034.123914
AAGCAGG114400.033.7773487
ATTCAGC37450.032.9165886
CTATAAC26800.032.7397582
GGTCGTT4700.032.3925977
CAGTACG9250.032.134669
AAGGTCG5650.032.0791669
GCAAAAG106300.031.379233
GAGCAAA108650.031.3017061
ATATTCA41200.030.976564
AGCAGGT50150.030.9367288
Produced by FastQC (version 0.11.5)