FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n01_2595_d12_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n01_2595_d12_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4435943
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA18650.061.041071
GCGAAAG21650.053.9218032
GAGCGAA12450.051.8348661
CGAAAGC26100.043.894793
GAGCAAA91400.041.570611
CAAAAGC97100.037.935074
GCAAAAG121500.030.3168353
AAAGCAG179600.023.8200196
AAAAGCA155900.023.5807845
GTAGAAA43400.023.3905091
GTATATA35650.023.3905051
AGCAAAA156500.023.2123872
AAGCAGG227400.019.2244877
TATATCG3401.5152655E-619.1937565
CTCCGAC5458.54925E-1118.623838145
GGCATAC31250.017.8637858
CTGATCG11050.017.7147189
TAGATGT39650.016.2758334
TAGAAAC59200.016.1677152
AATACCG3655.9603884E-515.89254955
Produced by FastQC (version 0.11.5)