FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n01_2595_d12_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n01_2595_d12_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2885190
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAAGC17650.047.2412873
AGCGAAA18450.045.5850751
GCGAAAG18400.045.315692
GAGCAAA62700.038.5068241
TCGTCGA900.003628413232.223931
CAAAAGC76400.031.222754
GAGCGAA13800.028.371071
GCAAAAG87800.027.0036093
GTTACGT4600.026.7948991
ACCGTCG1902.1948034E-422.8943658
AAGCAGG118800.022.7016537
AAAGCAG121550.022.6671646
GTATTAT28050.021.7123831
AGCAAAA108650.021.6881352
TACGGGG2806.0976854E-620.713957
AAAAGCA116800.020.547255
GTATATA19000.019.843161
TATAATA19150.019.3094542
TAGTACT8850.018.8431344
ATAAGAC11550.018.8324953
Produced by FastQC (version 0.11.5)