FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n01_2595_d10_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n01_2595_d10_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2833756
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAAAAGACTGATCAAGAGATTGAAGCAAGAAGGGGAAAAGAAACCTATA30280.10685464803603416No Hit
ACATAATGGCATACAAAAGACTGATCAAGAGATTGAAGCAAGAAGGGGAA28670.10117314264178003No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA13800.055.1679271
GAGCGAA7550.049.9381941
GCGAAAG15800.048.6435472
CGAAAGC17350.045.133683
CAAAAGC69750.043.659784
GAGCAAA70700.043.4833371
GCAAAAG79300.037.2132533
AGCAAAA97750.030.4860742
GTTACGT4200.027.621491
AAAAGCA114350.026.504315
AAAGCAG135700.025.2730546
GTAGAAA29550.024.5368581
TAGCGCG2401.6000504E-624.166246145
GGGTCGA2552.71082E-622.74439
TTACGTG4358.367351E-1121.6685832
GCTATAT11150.021.4592971
TATATAC18300.021.3953363
GAATCGC2104.3142476E-420.7161181
CAATAGG15250.020.4444311
GTATTGA11800.020.2772181
Produced by FastQC (version 0.11.5)