FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n01_2595_d10_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n01_2595_d10_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3017261
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA21500.052.270431
GCGAAAG21900.050.9838072
CGAAAGC22100.050.8504833
GAGCGAA11750.043.1939621
GAGCAAA73400.041.981291
CAAAAGC78250.038.452044
GCAAAAG88350.034.302473
AGCAAAA106250.028.250562
AAAGCAG138650.025.6758296
AAAAGCA130750.023.2342155
AAGCAGG158650.022.5293227
CAATAGG15650.020.3845541
GCGTATA1850.0050489719.5947449
TAGTACT7300.018.870634
GAGCTAT14600.018.374346
ACTCCGA6850.017.993731
GCTATTG16000.017.218888
AGCAGGT38500.016.7598678
GTATATA18150.016.3783471
CTAGTAC4551.3734316E-615.9347143
Produced by FastQC (version 0.11.5)