FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n01_2594_d8_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n01_2594_d8_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3262415
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAAGC13950.050.9358333
GCGAAAG14250.050.372312
AGCGAAA15050.047.6954421
TATATCG2151.2732926E-1137.0958865
TATAGCG2003.103502E-421.7516782
GAGCGAA7300.020.8580931
GTATATA27650.020.453971
GTACGGT2307.963085E-418.9145036
TAGTACC13850.016.7521954
GCTATAT11300.016.6829591
TACGGCT3504.16347E-516.5727084
TATATAC27300.016.4664733
GTATAAG12250.015.981071
GTCCTAT21350.015.6220551
TCTACGG2800.002971813815.5369122
ATATAGC11750.015.4267233
TTATACT25000.015.0811644
TATACTA12500.015.0811645
GGACCGT3406.2668684E-414.9276236
GTAATAC11700.014.8729433
Produced by FastQC (version 0.11.5)