FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n01_2594_d8_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n01_2594_d8_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3206432
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA25550.038.876431
CGAAAGC28900.034.8717423
GCGAAAG29100.034.133772
TATACGA3803.6379788E-1022.8942937
TACCGTA4505.456968E-1222.5565245
CGCGATA1750.003660546620.7142098
TATATAC22300.020.4829443
ATATAAG25000.020.3008731
ATATACG5555.456968E-1219.5954386
TATAATA19850.018.9932612
ATAAGAC31850.018.8940643
GAGCGAA11400.018.4437751
TATAAGA32150.018.2667272
TACGAAC4806.682967E-918.124659
TATTACA22600.017.9653762
GCTATAT11100.017.6358951
TACCATG17950.017.367357
GTATTAC16200.017.0069071
ATATACC19600.016.6461244
CTACCGT7051.0913936E-1116.4546094
Produced by FastQC (version 0.11.5)