FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n01_2594_d6_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n01_2594_d6_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3225656
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAAGC14550.048.336513
AGCGAAA14350.048.0011561
TATATCG2650.046.513935
GCGAAAG17250.041.191112
TATACTG19050.023.2174685
TTATACT14450.023.0818164
GAGCGAA8800.023.070411
TTATTCG2556.965087E-519.901415145
TCGTCTA3802.081797E-719.0793139
TATACTA5851.0913936E-1118.591545
ATATCGT8550.017.8087376
GTATATA17950.017.7733041
TATATAA16200.017.4548512
ATTATAC19050.017.1271083
GAGCAAA50850.016.9682081
ATATATA26500.016.9633772
TATATAC21750.016.3344063
CGATAGA3153.5065305E-416.1126691
CAACGCG2700.002332601216.111429
ATAGTAC19150.015.9018283
Produced by FastQC (version 0.11.5)